SSC: Sulfur acid is detected as sulfur compounds in sulphur compounds in sulphur compounds.

 

Experimental Principle

There are two main ways in which microorganisms produce hydrogen sulfide.

A:

Hydrogen sulfide is produced by cysteine reduction of protein components in the medium.Protein by microbial enzymes, [TJC STEEL]form of sulfur cysteine, the amino acid by cysteine desulfurase lose sulfur atoms, since joining hydrogen to form water molecules and hydrogen sulphide.

B:

Hydrogen sulfide is composed of an inorganic sulfur compound such as Thiosulfate;S2O32 - and sulfate;SO42 - or sulfite;SO32-, when the medium contains sodium thiosulfate, some microorganisms can reduce it to sulfite and release hydrogen sulfide.

In this experiment, SIM culture medium is used, containing protein and sodium thiosulfate as sulfur quality, another meridian sulfate;Fe(NH4)2SO4 [TJC STEEL]acts as an indicator of hydrogen sulphide (hydrogen sulphide is a colourless gas and is not easily detected. Ferrous ions of ammonium sulphate react with hydrogen sulphide to form black deposits of insoluble ferrous sulphide.)The medium was semi-solid with 0.3% AGAR to enhance anaerobic respiration. The motility of microorganisms was also observed. If motility was present, the growth of microorganisms diffused around the inoculation line, making the medium cloudy; if no motility was present, growth was restricted to the inoculation line.

 

Experimental Materials

Bacteria: Escherichia Coli, Enterobacter Aerogenes, Proteus Vulgaris

Culture Medium: Sim Agar Deep Tube3 Sticks

Equipment: Alcohol Lamp, Inoculation Needle

 

Experimental Steps

Each SIM AGAR Deep Tube was labeled with inoculation bacterium and group respectively.

The bacteria were punctured and inoculated [TJC STEEL]into the medium respectively by aseptic operation.

All the test tubes were cultured at 37℃ for 24-48 hours.

 

Results Analysis

Check all SIM media for black inoculations and record the results in the table.

According to the observation, whether [TJC STEEL]the bacteria have the ability to produce hydrogen sulfide or not.

Observe whether the microorganisms spread around the inoculation line and determine whether they are motile.